Then the total 25 engineered strains were cultured and induced in the respective conditions . the results show that the target products expressed in 3 strains of them are partly soluble and active , whils 11bl21 ( 0e3 )表达的包涵体蛋白进行了变性、复性研究,部分获得有活性的d一海因酶,酶活力约为0 . 90u / mgo